Successful crossings with early flowering transgenic poplar: interspecific crossings, but not transgenesis, promoted aberrant phenotypes in offspring.
Identifieur interne : 002035 ( Main/Exploration ); précédent : 002034; suivant : 002036Successful crossings with early flowering transgenic poplar: interspecific crossings, but not transgenesis, promoted aberrant phenotypes in offspring.
Auteurs : Hans Hoenicka [Allemagne] ; Denise Lehnhardt ; Ove Nilsson ; Dieter Hanelt ; Matthias FladungSource :
- Plant biotechnology journal [ 1467-7652 ] ; 2014.
Descripteurs français
- KwdFr :
- Arabidopsis (génétique), Facteurs temps (MeSH), Fleurs (croissance et développement), Fleurs (cytologie), Fleurs (génétique), Génotype (MeSH), Hybridation génétique (MeSH), Phénotype (MeSH), Plant (croissance et développement), Plant (cytologie), Plant (génétique), Pollen (croissance et développement), Pollen (cytologie), Pollen (génétique), Populus (croissance et développement), Populus (cytologie), Populus (génétique), Protéines d'Arabidopsis (génétique), Protéines d'Arabidopsis (métabolisme), Régulation de l'expression des gènes végétaux (MeSH), Saisons (MeSH), Sélection (MeSH), Techniques de transfert de gènes (MeSH), Température élevée (MeSH), Végétaux génétiquement modifiés (MeSH).
- MESH :
- croissance et développement : Fleurs, Plant, Pollen, Populus.
- cytologie : Fleurs, Plant, Pollen, Populus.
- génétique : Arabidopsis, Fleurs, Plant, Pollen, Populus, Protéines d'Arabidopsis.
- métabolisme : Protéines d'Arabidopsis.
- Facteurs temps, Génotype, Hybridation génétique, Phénotype, Régulation de l'expression des gènes végétaux, Saisons, Sélection, Techniques de transfert de gènes, Température élevée, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Arabidopsis (genetics), Arabidopsis Proteins (genetics), Arabidopsis Proteins (metabolism), Breeding (MeSH), Flowers (cytology), Flowers (genetics), Flowers (growth & development), Gene Expression Regulation, Plant (MeSH), Gene Transfer Techniques (MeSH), Genotype (MeSH), Hot Temperature (MeSH), Hybridization, Genetic (MeSH), Phenotype (MeSH), Plants, Genetically Modified (MeSH), Pollen (cytology), Pollen (genetics), Pollen (growth & development), Populus (cytology), Populus (genetics), Populus (growth & development), Seasons (MeSH), Seedlings (cytology), Seedlings (genetics), Seedlings (growth & development), Time Factors (MeSH).
- MESH :
- chemical , genetics : Arabidopsis Proteins.
- cytology : Flowers, Pollen, Populus, Seedlings.
- genetics : Arabidopsis, Flowers, Pollen, Populus, Seedlings.
- growth & development : Flowers, Pollen, Populus, Seedlings.
- chemical , metabolism : Arabidopsis Proteins.
- Breeding, Gene Expression Regulation, Plant, Gene Transfer Techniques, Genotype, Hot Temperature, Hybridization, Genetic, Phenotype, Plants, Genetically Modified, Seasons, Time Factors.
Abstract
In forest tree species, the reproductive phase is reached only after many years or even decades of juvenile growth. Different early flowering systems based on the genetic transfer of heat-shock promoter driven flowering-time genes have been proposed for poplar; however, no fertile flowers were reported until now. Here, we studied flower and pollen development in both HSP::AtFT and wild-type male poplar in detail and developed an optimized heat treatment protocol to obtain fertile HSP::AtFT flowers. Anthers from HSP::AtFT poplar flowers containing fertile pollen grains showed arrested development in stage 12 instead of reaching phase 13 as do wild-type flowers. Pollen grains could be isolated under the binocular microscope and were used for intra- and interspecific crossings with wild-type poplar. F1-seedlings segregating the HSP::AtFT gene construct according to Mendelian laws were obtained. A comparison between intra- and interspecific crossings revealed that genetic transformation had no detrimental effects on F1-seedlings. However, interspecific crossings, a broadly accepted breeding method, produced 47% seedlings with an aberrant phenotype. The early flowering system presented in this study opens new possibilities for accelerating breeding of poplar and other forest tree species. Fast breeding and the selection of transgene-free plants, once the breeding process is concluded, can represent an attractive alternative even under very restrictive regulations.
DOI: 10.1111/pbi.12213
PubMed: 24975279
Affiliations:
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first="Ove" last="Nilsson">Ove Nilsson</name></author><author><name sortKey="Hanelt, Dieter" sort="Hanelt, Dieter" uniqKey="Hanelt D" first="Dieter" last="Hanelt">Dieter Hanelt</name></author><author><name sortKey="Fladung, Matthias" sort="Fladung, Matthias" uniqKey="Fladung M" first="Matthias" last="Fladung">Matthias Fladung</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:24975279</idno><idno type="pmid">24975279</idno><idno type="doi">10.1111/pbi.12213</idno><idno type="wicri:Area/Main/Corpus">002102</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002102</idno><idno type="wicri:Area/Main/Curation">002102</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002102</idno><idno type="wicri:Area/Main/Exploration">002102</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Successful crossings with early 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Different early flowering systems based on the genetic transfer of heat-shock promoter driven flowering-time genes have been proposed for poplar; however, no fertile flowers were reported until now. Here, we studied flower and pollen development in both HSP::AtFT and wild-type male poplar in detail and developed an optimized heat treatment protocol to obtain fertile HSP::AtFT flowers. Anthers from HSP::AtFT poplar flowers containing fertile pollen grains showed arrested development in stage 12 instead of reaching phase 13 as do wild-type flowers. Pollen grains could be isolated under the binocular microscope and were used for intra- and interspecific crossings with wild-type poplar. F1-seedlings segregating the HSP::AtFT gene construct according to Mendelian laws were obtained. A comparison between intra- and interspecific crossings revealed that genetic transformation had no detrimental effects on F1-seedlings. However, interspecific crossings, a broadly accepted breeding method, produced 47% seedlings with an aberrant phenotype. The early flowering system presented in this study opens new possibilities for accelerating breeding of poplar and other forest tree species. Fast breeding and the selection of transgene-free plants, once the breeding process is concluded, can represent an attractive alternative even under very restrictive regulations. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24975279</PMID><DateCompleted><Year>2015</Year><Month>06</Month><Day>08</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1467-7652</ISSN><JournalIssue CitedMedium="Internet"><Volume>12</Volume><Issue>8</Issue><PubDate><Year>2014</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Plant biotechnology journal</Title><ISOAbbreviation>Plant Biotechnol J</ISOAbbreviation></Journal><ArticleTitle>Successful crossings with early flowering transgenic poplar: interspecific crossings, but not transgenesis, promoted aberrant phenotypes in offspring.</ArticleTitle><Pagination><MedlinePgn>1066-74</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/pbi.12213</ELocationID><Abstract><AbstractText>In forest tree species, the reproductive phase is reached only after many years or even decades of juvenile growth. Different early flowering systems based on the genetic transfer of heat-shock promoter driven flowering-time genes have been proposed for poplar; however, no fertile flowers were reported until now. Here, we studied flower and pollen development in both HSP::AtFT and wild-type male poplar in detail and developed an optimized heat treatment protocol to obtain fertile HSP::AtFT flowers. Anthers from HSP::AtFT poplar flowers containing fertile pollen grains showed arrested development in stage 12 instead of reaching phase 13 as do wild-type flowers. Pollen grains could be isolated under the binocular microscope and were used for intra- and interspecific crossings with wild-type poplar. F1-seedlings segregating the HSP::AtFT gene construct according to Mendelian laws were obtained. A comparison between intra- and interspecific crossings revealed that genetic transformation had no detrimental effects on F1-seedlings. However, interspecific crossings, a broadly accepted breeding method, produced 47% seedlings with an aberrant phenotype. The early flowering system presented in this study opens new possibilities for accelerating breeding of poplar and other forest tree species. Fast breeding and the selection of transgene-free plants, once the breeding process is concluded, can represent an attractive alternative even under very restrictive regulations. </AbstractText><CopyrightInformation>© 2014 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hoenicka</LastName><ForeName>Hans</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Thünen Institute of Forest Genetics, Grosshansdorf, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lehnhardt</LastName><ForeName>Denise</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Nilsson</LastName><ForeName>Ove</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Hanelt</LastName><ForeName>Dieter</ForeName><Initials>D</Initials></Author><Author 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Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005838" MajorTopicYN="N">Genotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006358" MajorTopicYN="N">Hot Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006824" MajorTopicYN="N">Hybridization, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011058" MajorTopicYN="N">Pollen</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000166" 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breeding</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>03</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>04</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>05</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>7</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>7</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>6</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24975279</ArticleId><ArticleId IdType="doi">10.1111/pbi.12213</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country></list><tree><noCountry><name sortKey="Fladung, Matthias" sort="Fladung, Matthias" uniqKey="Fladung M" first="Matthias" last="Fladung">Matthias Fladung</name><name sortKey="Hanelt, Dieter" sort="Hanelt, Dieter" uniqKey="Hanelt D" first="Dieter" last="Hanelt">Dieter Hanelt</name><name sortKey="Lehnhardt, Denise" sort="Lehnhardt, Denise" uniqKey="Lehnhardt D" first="Denise" last="Lehnhardt">Denise Lehnhardt</name><name sortKey="Nilsson, Ove" sort="Nilsson, Ove" uniqKey="Nilsson O" first="Ove" last="Nilsson">Ove Nilsson</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Hoenicka, Hans" sort="Hoenicka, Hans" uniqKey="Hoenicka H" first="Hans" last="Hoenicka">Hans Hoenicka</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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